Unfolding Properties of Tryptophan-containing α-Subunits of the Escherichia coli Tryptophan Synthase
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چکیده
منابع مشابه
Interactions of tryptophan synthetase subunits in Escherichia coli containing mutationally altered beta2 subunits.
The (Y and /I2 subunits of tryptophan synthetase are specified by the A and B genes, respectively, of the trp operon in Escherichia coli. Missense mutations in trpB result in catalytically inactive /I2 subunits. In some cases, these mutations also diminish the catalytic and /I2 subunit stimulating activities of the (Y subunit in cell extracts. Analysis of repressor-negative (trpR) strains havin...
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The (Y and /I2 subunits of tryptophan synthetase are specified by the A and B genes, respectively, of the trp operon in Escherichia coli. Missense mutations in trpB result in catalytically inactive /I2 subunits. In some cases, these mutations also diminish the catalytic and /I2 subunit stimulating activities of the (Y subunit in cell extracts. Analysis of repressor-negative (trpR) strains havin...
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Coenzyme Derivatives, Pyridoxal 5'-Phosphate, Tryptophan Synthase The interaction of the coenzyme analogues pyridoxal (A), pyridoxine 5'-phosphate (B), pyridoxic acid 5'-phosphate (Q and N-phosphopyridoxyl-L-serine (D) with both the isolated apo ß2 subunit and the native <x2 apo ß2 bienzyme complex of tryptophan synthase from Escherichia coli has been investigated using enzyme kinetics and CD s...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1995
ISSN: 0021-9258
DOI: 10.1074/jbc.270.47.28177